IIIrd B.Sc. Biology,Genetic Engineering and Immunology Question Paper

THREE YEAR B.Sc. DEGREE EXAMINATION, APRIL/MAY 2014.

THIRD YEAR EXAMINATION

Part II - Biotechnology

Paper III : MOLECULAR BIOLOGY, GENETIC ENGINEERING AND IMMUNOLOGY

Time: Three hours                                                                                Maximum 100 marks

SECTION -- I (4 x 15 = 60 marks)

Answer ALL questions.

1. (a) Describe the replication mechanism of DNA?

(or)

(b) Describe the structure of telomeric DNA and mention its significance

2. (a) Write a general account of gene expression

(or)

2. (a) Write a general account of gene expression

(b) Describe in detail about the features of genetic code

3. (a) Write a general account of vectors?

(or)

(b) Describe about the enzymes used in cloning?

4. (a) Explain hypersensitivity in detail ?

(or)

(b) Describe the structure and functions of immunoglobulins?

SECTION II -  (8 x 5-40 marks)

Answer any EIGHT questions

5. Messelson and Stabl experiment

6. DNA damage

7. Nick translation

8. Wobble hypothesis

9. Structure of promoter

10. Significance of o (sigma) factor

11. cDNA-rDNA

12. PCR

13. Western blotting

14. Haptens

15. Agglutination

16. Antigen-Antibody reaction