M.Sc. DEGREE EXAMINATION, OCTOBER / NOVEMBER 2015.
FIRST SEMESTER
PAPER IV- TOOLS AND TECHNIQUES IN BIOLOGY
Maximum : 75 marks Time : Three hours
(No additional Sheet will be supplied)
PART - A
(5x3=15 marks)
- Answer any FIVE questions
- Each question carries 3 marks.
- Each answer should not exceed 1 page
1. Electron microscope.
2. Spectrophotometer
3. Ion exchange chromotography
4. Applications of radio isotopes in biological sciences
5. Microtome
6. ELISA
7. Cell viability
8. Cell harvesting methods
PART - B (4x 15 =60 marks)
- Answer ALL questions
- Each questions carries 15 marks.
- Each answer should not exceed 6 pages.
9. Write an account on types of microscope, its principle and applications.
Or
10. Describe the operative principle of Ultra centrifuge and explain its uses in biology
11. Differentiate principle and applications of paper chromatography and thin layer chromatography.
Or
12. Enumerate the functional features of radio isotopes and mass isotope techniques.
13. Explain the histological techniques that are used for identification of cell organelles
Or
14. Give a brief account on Blotting techniques and their applications with suitable examples.
15. write an account on the design of tissue culture laboratory and equipment required.
Or
16. Describe composition of culture media, their preparation and sterilization.
