M.Sc. DEGREE EXAMINATION, NOVEMBER 2016.
THIRD SEMESTER
Zoology
Paper II -- MOLECULAR BIOLOGY
Time: Three hours Maximum 75 marks
(No additional sheet will be supplied)
PART A -(5 x 3 = 15 marks)
Answer any FIVE questions.
Each question carries 3 marks.
Each answer should not exceed 1 page.
1. Central dogma of Molecular Biology
2. One gene one enzyme hypothesis.
3. Reverse Transcriptase
4. DNA Polymerase I
5. Ribozymes
6. Inhibitors of translation
7. Tryptophan operon
8. Sanger dideoxy method of sequencing.
PART B -(4 x 5 = 60 marks)
Answer ALL questions.
Each question carries 15 marks
Each answer should not exceed 6 pages.
9. Give a detailed account of Watson and Crick model of DNA and properties of DNA.
10. Write a note on :
(a) Nuclear organisation and
(b) Mitochondrial Genome
(a) Nuclear organisation and
(b) Mitochondrial Genome
11. Write in detail about different DNA repair mechanisms and add a note on their significance in maintaining DNA integrity.
Or
12. Write a detail note on various enzymes involved in DNA replication with special emphasis on their function.
13. Write a note on different types of RNA and their functions
Or
14. write a detailed note on
(a) Transcription and
(b) Post transcriptional modifications.
(a) Transcription and
(b) Post transcriptional modifications.
15. Write detail note on gene regulation with special reference to Lac operon.
Or
16. Write a detailed note on construction of cDNA libraries.
